马三垣
西南大学 研究员，硕士生导师
国家优青（2021入选）
重庆英才青年拔尖人才（2020入选）
学习工作经历
2004.09—2008.07 西南大学生物技术学院蚕学专业，学士
2008.09—2014.12 西南大学特种经济动物饲养专业，博士（硕博连读）
2015.01—2017.03 西南大学，师资博士后
2017.05—2018.06 西南大学，助理研究员
2018.07—2021.06 西南大学，副教授
2021.07至今 西南大学，研究员
2022.01至今 西部（重庆）科学城种质创制大科学中心，家蚕种质创制团队科研人员
获奖及荣誉
2015 重庆市优秀博士学位论文
2018 重庆市自然科学二等奖（家蚕分子育种关键技术与素材创新）
2020 首届川渝科学技术大会优秀论文二等奖（全基因组CRISPR筛选揭示家蚕生长必需 基因以及生物/非生物逆境抗性基因）
学术兼职
2020至今：Frontiers in Molecular Bioscience (IF=5.246)，副主编（Associate Editor）
2020至今：Frontiers in Genetics (IF=4.599)，副主编（Associate Editor）
2020至今：Frontiers in Ecology and Evolution (IF=4.171)，副主编（Associate Editor）
2020至今：Agronomy (IF=3.417)，专刊编辑（Special Issue Editor）
2021至今：Journal of Visualized Experiments (IF=1.355)，专刊编辑（Special Issue Editor）
2020至今：Frontiers in Genetics (IF=4.599)，编委（Review Editor）
2017至今：中国昆虫学会，基因组专业委员会，委员
2017至今：中国昆虫学会，青年工作委员会，委员
2014至今：国际昆虫转基因协作组织（ITGRN），会员
长期担任10余个SCI期刊的审稿人：Journal of Pest Science、Insect Biochemistry and Molecular Biology、BMC Biotechnology、Biotechnology and Applied Biochemistry 、Protein & Peptide Letters 、Archives of Insect Biochemistry & Physiology、Current Gene Therapy、Journal of Insect Science、Scientific Reports、PLoS One等
研究领域与方向
1．基于全基因组CRISPR 文库的规模化遗传筛选与种质创制
2．基因组编辑等前沿遗传操作技术的开发及其在家蚕中的应用。
3．3D基因组调控基因表达的分子机制及其在家蚕丝蛋白基因超高量表达中的机制。
4．家蚕丝腺的遗传重塑、改良利用与新型丝纤维的创制。
主要科学贡献
建立了基于ZFN、TALEN和Cas9的多套家蚕基因组编辑技术体系，突破了定点突变、靶向调控、表观修饰、单碱基编辑、精准原位标记等DNA操作技术，构建了世界首个家蚕全基因组编辑文库，相关论文发表于国际顶级期刊Genome Research（生物学top期刊，五年IF=11.638，Nature指数期刊），并被主编选为封面故事和当期重点推介文章，在世界范围内引起广泛关注。累积在Genome Research、Cell Research、Autophagy、J Hazard Mater、J Genet Genomics、Insect Biochem Mol Biol等杂志发表论文50余篇，被引用1000余次，施引论文包括发表于Nature review系列、Annual review系列等顶级综述论文，及发表于Nature communications、PNAS、Nucleic Acids Res等知名权威期刊的研究论文等；获得国际授权专利1项和国家发明专利10余项；受邀到美国、英国、日本等国的知名研究机构做学术报告10余次；相关成果多次被《重庆新闻联播》（2014，2020）、《重庆日报》、《重庆晚报》、《科学网》、等媒体视频/文字报道，受到业界的高度好评和社会的广泛关注。
发表论文
(by 15-12-2020, co-first author indicated by #, and corresponding authors indicated by *):
Research papers mention in the journal cover or special issue:
Research papers mention in the journal cover or special issue:
Publication list:
55) Lu W, Wang R, Wang P, Ma S*, Xia Q*. Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines. Insects. 2021 Dec 1;12(12):1081. (IF=2.769)
54) Lu W, Lan X, Zhang T, Sun H, Ma S*, Xia Q*. Precise Characterization of Bombyx mori Fibroin Heavy Chain Gene Using Cpf1-Based Enrichment and Oxford Nanopore Technologies. Insects. 2021 Sep 16;12(9):832. (IF=2.769)
53) Hu W, Wang X, Ma S, Peng Z, Cao Y, Xia Q*. CRISPR-Mediated Endogenous Activation of Fibroin Heavy Chain Gene Triggers Cellular Stress Responses in Bombyx mori Embryonic Cells. Insects. 2021 Jun 13;12(6):552. (IF=2.769)
52) Ma S*, Wang A, Chen X, Zhang T, Xing W, Xia Q*. Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori. CRISPR J. 2021 Jun;4(3):371-380. (IF=6.071)
51) Liu Y, Chang J, Yang C, Zhang T, Chen X, Shi R, Liang Y, Xia Q*, Ma S*. Genome-wide CRISPR-Cas9 screening in Bombyx mori reveals the toxicological mechanisms of environmental pollutants, fluoride and cadmium. J Hazard Mater. 2021 May 15; 410:124666. (IF=10.588)
50) Liu Y, Yang C, Sun L, Wang A, Lan X, Xu W, Liang Y, Ma S*, Xia Q*. In-depth transcriptome unveils the cadmium toxicology and a novel metallothionein in silkworm. Chemosphere. 2021 Jun; 273:128522. (IF=7.086)
49) Hua X, Xu W, Ma S, Xia Q*. STING-dependent autophagy suppresses Nosema bombycis infection in silkworms, Bombyx mori. Dev Comp Immunol. 2021 Feb;115:103862. (IF=3.636)
48) Liu Y, Liang Y, Yang C, Shi R, Lu W, Wang X, Wang R, Xia Q*, Ma S*. A deep insight into the transcriptome of midgut and fatbody reveals the toxic mechanism of fluoride exposure in silkworm. Chemosphere. 2021 Jan;262:127891. (IF=7.086)
47) Chang J, Chen X, Zhang T, Wang R, Wang A, Lan X, Zhou Y, Ma S*, Xia Q*. The novel insight into the outcomes of CRISPR/Cas9 editing intra- and inter-species. Int J Biol Macromol. 2020 Jul 8; 163:711-717. (IF=5.162)
46) Chang J#, Wang R#, Yu K, Zhang T, Chen X, Liu Y, Shi R, Wang X, Xia Q*, Ma S*. Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses in Bombyx mori. Genome Res. 2020 May; 30(5):757-767. (IF=11.093, cover story)
45) Liu Y, Li Y, Liang Y, Wang T, Yang C, Ma S*, Xia Q*. Comparative analysis of genome editing systems, Cas9 and BE3, in silkworms. Int J Biol Macromol. 2020 Apr 25; 158:486-492. (IF=5.162)
44) Wang W, Wang X, Luo C, Pu Q, Yin Q, Xu L, Peng X, Ma S,Xia Q, Liu S*. Let-7 microRNA is a critical regulator in controlling the growth and function of silk gland in the silkworm. RNA Biol. 2020 May; 17(5):703-717. (IF=5.35)
43) Chang J, Chen X, Wang R, Shi R, Wang X, Lu W, Ma S*, Xia Q*. High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression. Molecules. 2020 Jan 15; 25(2):353. (IF=3.267)
42) Xing W, Ma S*, Liu Y, Xia Q*. CRISPR/dCas9-mediated imaging of endogenous genomic loci in living Bombyx mori cells. Insect Sci. 2019 Sep 1. 27:1360-1364. (IF=2.791)
41) Shi R, Ma S,He T, Peng J, Zhang T, Chen X, Wang X, Chang J, Xia Q, Zhao P*. Deep Insight into the Transcriptome of the Single Silk Gland of Bombyx mori. Int J Mol Sci. 2019 May 20; 20(10):2491. (IF=4.556)
40) Wang X, Ma S,Liu Y, Lu W, Sun L, Zhao P, Xia Q*. Transcriptional repression of endogenous genes in BmE cells using CRISPRi system. Insect Biochem Mol Biol. 2019 Aug; 111:103172. (IF=3.827)
39) Liu Y, Ma S, Chang J, Zhang T, Chen X, Liang Y, Xia Q*. Programmable targeted epigenetic editing using CRISPR system in Bombyx mori. Insect Biochem Mol Biol. 2019 Jul; 110:105-111. (IF=3.827)
38) Ma S*, Zhang J, Lu W, Liu Y, Xia Q. SAA-Cas9: A tunable genome editing system with increased bio-safety and reduced off-target effects. J Genet Genomics. 2019 Mar 20; 46(3):145-148. (IF=5.065)
37) Dong Z, Guo K, Zhang X, Zhang T, Zhang Y, Ma S, Chang H, Tang M, An L, Xia Q, Zhao P*. Identification of Bombyx mori sericin 4 protein as a new biological adhesive. Int J Biol Macromol. 2019 Jul 1; 132:1121-1130. (IF=5.162)
36) Shen G, Wu J, Wang Y, Liu H, Zhang H, Ma S, Peng C, Lin Y, Xia Q*. The expression of ecdysteroid UDP-glucosyltransferase enhances cocoon shell ratio by reducing ecdysteroid titre in last-instar larvae of silkworm, Bombyx mori. Sci Rep. 2018 Dec 7; 8(1):17710. (IF=3.998)
35) Wang X, Ma S, Chang J, Shi R, Wang R, Zhao P, Xia Q*. Programmable activation of Bombyx gene expression using CRISPR/dCas9 fusion systems. Insect Sci. 2019 Dec; 26(6):983-990. (IF=2.791, invited article for special issue)
34) Jia L, Ma S, Zhang T, Xing W, Liu Y, Li Y, Chen X, Xia Q*. Enhanced Bombyx genome editing via Cas9 ribonucleoprotein injection. Insect Sci. 2019 Dec; 26(6): 1059-1062. 30084531. (IF=2.791, invited letter for special issue)
33) Ma S*, Liu Y, Lu W, Wang X, Sun L, Yu K, Xia Q*. Targeted activation of BmCyclinE in Bombyx mori using designer TAL effectors. Insect Sci. 2019 Dec; 26(6):1055-1058. (IF=2.791, invited letter for special issue)
32) Ma S*, Smagghe G*, Xia Q*. Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericulture industry. Insect Sci. 2019 Dec; 26(6):964-972. (IF=2.791, invited review for special issue)
31) Li Y#, Ma S#, Sun L, Zhang T, Chang J, Lu W, Chen X, Liu Y, Wang X, Shi R, Zhao P, Xia Q*. Programmable Single and Multiplex Base-Editing in Bombyx mori Using RNA-Guided Cytidine Deaminases. G3 (Bethesda). 2018 May 4; 8(5):1701-1709. (IF=2.781)
30) Peng Z, Liu C, Zhang L, Li W, Hu W, Ma S, Xia Q*. A Simple Method for the Cross-Section Area Determination of Single Profiled Fibers and Its Application. Microsc Microanal. 2018 Feb; 24(1):17-28. (IF=3.414)
29) Ma S, Xia Q*. Genetic breeding of silkworms: from traditional hybridization to molecular design. Yi Chuan. 2017 Nov 20; 39(11):1025-1032. (IF=0)
28) Liu Y#, Ma S#, Chang J, Zhang T, Wang X, Shi R, Zhang J, Lu W, Liu Y, Xia Q*. Tissue-specific genome editing of laminA/C in the posterior silk glands of Bombyx mori. J Genet Genomics. 2017 Sep 20; 44(9):451-459. (IF=5.065, invited article for special issue)
27) Ma S#, Xia X#, Li Y, Sun L, Liu Y, Liu Y, Wang X, Shi R, Chang J, Zhao P, Xia Q*. Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1. Mol Genet Genomics. 2017 Aug; 292(4):823-831. (IF=2.797)
26)Ma S#, Liu Y#, Liu Y, Chang J, Zhang T, Wang X, Shi R, Lu W, Xia X, Zhao P, Xia Q*. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites. Insect Biochem Mol Biol. 2017 Apr; 83:13-20. (IF=3.827)
25) Taning CNT, Van Eynde B, Yu N,Ma S, Smagghe G*. CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns. J Insect Physiol. 2017 Apr; 98:245-257. (IF=2.246)
24) Wang D, Zhang Y, Dong Z, Guo P, Ma S, Guo K, Xia Q, Zhao P*. Serine protease P-IIc is responsible for the digestion of yolk proteins at the late stage of silkworm embryogenesis. Insect Biochem Mol Biol. 2016 Jul ;74:42-9. (IF=3.827)
23) Lin P, Cheng T,Ma S, Gao J, Jin S, Jiang L, Xia Q*. Bacillus bombysepticus α-Toxin Binding to G Protein-Coupled Receptor Kinase 2 Regulates cAMP/PKA Signaling Pathway to Induce Host Death. PLoS Pathog. 2016 Mar 29;12(3):e1005527. (IF=6.218)
22) Duan J, Meng X,Ma S, Wang F, Guo H, Zhang L, Zhao P, Kan Y, Yao L, Xia Q*. The C-terminus of DSX(F5) protein acts as a novel regulatory domain in Bombyx mori. Transgenic Res. 2016 Aug; 25(4):491-7. (IF=1.856)
21) Hu C, Wang F, Ma S, Li X, Song L, Hua X, Xia Q*. Suppression of intestinal immunity through silencing of TCTP by RNAi in transgenic silkworm, Bombyx mori. Gene. 2015 Dec 10; 574(1):82-7. (IF=2.984)
20) Wang X, Zhao P, Li Y, Yi Q, Ma S, Xie K, Chen H, Xia Q*. Modifying the Mechanical Properties of Silk Fiber by Genetically Disrupting the Ionic Environment for Silk Formation. Biomacromolecules. 2015 Oct 12 ;16(10):3119-25. (IF=6.092)
19) Chen Q, Ma Z, Wang X, Li Z, Zhang Y, Ma S, Zhao P, Xia Q*. Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues. Funct Integr Genomics. 2015 Sep; 15(5):611-37. (IF=3.058)
18) Wang F, Wang R, Wang Y, Xu H, Yuan L, Ding H, Ma S, Zhou Y, Zhao P, Xia Q*. Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm. Mol Genet Genomics. 2015 Jun; 290(3):1181-9. (IF=2.797)
17) Ma S#, Shi R#, Wang X, Liu Y, Chang J, Gao J, Lu W, Zhang J, Zhao P, Xia Q*. Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor. Sci Rep. 2014 Oct 31; 4:6867. (IF=3.998)
16) Gao J, Wang G, Ma S,Xie X, Wu X, Zhang X, Wu Y, Zhao P, Xia Q*. CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum. Plant Mol Biol. 2015 Jan;87(1-2):99-110. (IF=3.302)
15) Wang F, Xu H, Wang Y, Wang R, Yuan L, Ding H, Song C, Ma S, Peng Z, Peng Z, Zhao P, Xia Q*. Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application. Acta Biomater. 2014 Dec; 10(12):4947-4955. (IF=7.242)
14) Xu H, Liu Y, Wang F, Yuan L, Wang Y, Ma S,Bene? H, Xia Q*. Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori. Transgenic Res. 2014 Aug;23(4):669-77. (IF=1.856)
13) Liu Y#, Ma S#, Wang X, Chang J, Gao J, Shi R, Zhang J, Lu W, Liu Y, Zhao P, Xia Q*. Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect Biochem Mol Biol. 2014 Jun; 49:35-42. (IF=3.827)
12) Ma S#, Chang J#, Wang X, Liu Y, Zhang J, Lu W, Gao J, Shi R, Zhao P, Xia Q*. CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci Rep. 2014 Mar 27; 4:4489. (IF=3.998)
11) Ma S, Wang X, Liu Y, Gao J, Zhang S, Shi R, Chang J, Zhao P, Xia Q*. Multiplex genomic structure variation mediated by TALEN and ssODN. BMC Genomics. 2014 Jan 18;15(1):41. (IF=3.594)
10) Duan J, Xu H, Guo H, O'Brochta DA, Wang F, Ma S, Zhang L, Zha X, Zhao P, Xia Q*. New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyx mori. PLoS One. 2013 Nov 7;8(11):e79703. (IF=2.74)
9) Wang F#, Ma S#, Xu H, Duan J, Wang Y, Ding H, Liu Y, Wang X, Zhao P, Xia Q*. High?efficiency system for construction and evaluation of customized TALENs for silkworm genome editing. Mol Genet Genomics. 2013 Dec;288(12):683-90. (IF=2.797)
8)Ma S#, Wang X#, Fei J, Liu Y, Duan J, Wang F, Xu H, Zhao P, Xia Q*. Genetic marking of sex using a W chromosome-linked transgene. Insect Biochem Mol Biol. 2013 Dec;43(12):1079-86. (IF=3.827)
7) Tian L, Ma L, Guo E, Deng X, Ma S, Xia Q, Cao Y*, Li S*. 20-Hydroxyecdysone upregulates Atg genes to induce autophagy in the Bombyx fat body. Autophagy. 2013 Aug; 9(8):1172-87. (IF=9.77)
6) Deng D, Xu H, Wang F, Duan X, Ma S, Xiang Z, Xia Q*. The promoter of Bmlp3 gene can direct fat body-specific expression in the transgenic silkworm, Bombyx mori. Transgenic Res. 2013 Oct; 22(5):1055-63. (IF=1.856)
5) Wang F, Xu H, Yuan L,Ma S, Wang Y, Duan X, Duan J, Xiang Z, Xia Q*. An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Transgenic Res. 2013 Oct; 22(5):925-38. (IF=1.856)
4) Duan J, Xu H, Ma S, Guo H, Wang F, Zhao P, Xia Q*. Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori). Transgenic Res. 2013 Jun;22(3):607-19. (IF=1.856)
3) Duan J, Xu H, Wang F, Ma S, Zha X, Guo H, Zhao P, Xia Q*. Novel female-specific trans-spliced and alternative splice forms of dsx in the silkworm Bombyx mori. Biochem Biophys Res Commun. 2013 Feb 15; 431(3):630-5. (IF=2.985)
2) Ma S, Zhang S, Wang F, Liu Y, Liu Y, Xu H, Liu C, Lin Y, Zhao P, Xia Q*. Highly efficient and specific genome editing in silkworm using custom TALENs. PLoS One. 2012; 7(9):e45035. (IF=2.74, special collection and highlighted by the journal with top 1% cited)
1) Ma L#, Xu H#, Zhu J, Ma S, Liu Y, Jiang RJ, Xia Q*, Li S*. Ras1(CA) overexpression in the posterior silk gland improves silk yield. Cell Res. 2011 Jun; 21(6):934-43. (IF=20.507)
联系方式
邮箱：masy@swu.edu.cn