郑雪莲
电子科技大学生命科学与技术学院，副教授
2011.07-至今 电子科技大学生命科学与技术学院，副教授，植物基因组定向编辑；
2007.07–2011.06 电子科技大学生命科学与技术学院，讲师，研究能源专用甘薯的选育与甘薯抗逆的分子机制；
2003.07–2004.10 美国康涅狄格大学(University of Connecticut)农学院植物系，访问学者，研究基于“gene-deletor”技术的转基因植物生物安全性控制；
2002.09–2007.06 西南大学生物技术中心与美国康涅狄格大学农学院联合培养计划，作物遗传育种专业，博士，研究内源生长素含量对棉花纤维发育的影响； 2000.09–2002.06 西南农业大学生物技术中心，生物化学与分子生物学专业，硕士研究生，研究甘薯及虫生真菌的遗传转化体系；
1996.09–2000.06 西南师范大学生命科学学院，生物教育专业，学士。
个人简介
植物基因组定向编辑技术 植物糖代谢及淀粉合成的机制 利用基因组定向编辑技术改良作物性状
科研项目
国家自然科学基金面上项目：
基于水稻支链淀粉生物合成途径多基因定向聚合突变体库的淀粉合成机制研究（编号：32072045，2021-2024）
四川省科技计划项目-应用基础
基于CRISPR-Cas12a基因编辑系统的高产优质高海拔特色粳稻创制（编号：21YYJC3034，2021-2023）
国家自然科学基金地区项目
基于CRISPR-Cas基因编辑系统的高海拔冷凉粳稻多基因定向聚合育种体系构建及高产优质材料创制（编号：31960423，2020-2023）
国家转基因重大专项子课题
基于大数据挖掘的水稻CRISPR-Cas精准基因组编辑新系统构建及应用（2019-2020）
国家自然科学基金面上项目
基于合成生物学的丹参酚酸类成分基因尺度代谢网络调控机制研究（编号：81872957，2019-2022）
国家自然科学基金面上项目
马铃薯TALEN基因组定向突变技术体系的建立及在淀粉合成机理研究上的应用（编号：31371682，2014-2017）
国家转基因重大专项子课题
小麦高效基因组定向编辑技术体系的建立及应用（2014-2016）
国家自然科学基金重点项目子课题
落叶松干细胞发育关键MicroRNAs功能分析及其TALEN定向突变体创制（编号：31330017，2014-2018）
国家自然科学基金面上项目
靶向核酸酶技术体系在小麦基因组定位修饰中的建立及应用研究（编号：31271420，2013-2016）
国家自然科学基金青年科学基金项目
维管组织特异表达蔗糖转运蛋白基因SUT对甘薯淀粉合成的影响（编号：31000735, 2011-2013）
国家甘薯产业技术体系（nycytx-16-B-4）
子课题：甘薯淀粉合成相关基因的克隆与遗传转化研究（2009-2012）
国家自然科学基金面上项目
两类植物抗菌肽氧化重折叠机制及影响因素的比较研究（编号：20973034, 2010-2012）
国家自然科学基金面上项目：
两类植物抗菌蛋白LJAMP1和LJAMP2在植物抗病中的作用（编号：30771388, 2008-2010）
国家自然科学基金科学基金重点项目
利用激素合成与传导基因改良棉花的纤维品质与产量（编号：30530490，2006-2009）
国家重点基础研究发展计划“973”项目
棉花纤维品质性状相关功能基因的克隆与鉴定（编号：2004CB117300，2004-2009）
Controlled removal of GM genes from the pollen and seed of aspen plants. US-DOE/CPBR. （从转基因植物的花粉和种子中去除转基因成分的研究，美国能源部资助项目）
Development of “super-sterile” cultivars for economically important invasive ornamental plants. USDA-NRI.（通过“超级不育”技术控制外来入侵物种的扩散和传播，美国农业部资助项目）
研究成果
1. Qiurong Ren , Simon Sretenovic , Shishi Liu , Xu Tang , Lan Huang , Yao He , Li Liu , Yachong Guo , Zhaohui Zhong , Guanqing Liu , Yanhao Cheng , Xuelian Zheng , Changtian Pan , Desuo Yin , Yingxiao Zhang , Wan-Feng Li , Liwang Qi , Chenghao Li , Yong Zhang, Yiping Qi. PAM-less plant genome editing using a CRISPR-SpRY toolbox. Nature Plant. 2020,
2. Xuelian Zheng, Caiyan Qi, Lijia Yang, Quan Quan, Binglin Liu, Zhaohui Zhong, Xu Tang, Tingting Fan, Jianping Zhou and Yong Zhang. The improvement of CRISPR-Cas9 system with ubiquitin-associated domain fusion for efficient plant genome editing. Front. Plant Sci, 2020, 11:621. doi:10.3389/fpls.2020.00621.
3. Xu Tang, Simon Sretenovic, Qiurong Ren, Xinyu Jia, Mengke Li, Tingting Fan, Desuo Yin, Shuyue Xiang, Yachong Guo, Li Liu, Xuelian Zheng, Yiping Qi, Yong Zhang. Plant prime editors enable precise gene editing in rice cells. Molecular Plant. 2020, 13:667-670.
4. Meiling Ming, Qiurong Ren, Changtian Pan, Yao He, Yingxiao Zhang, Shishi Liu, Zhaohui Zhong, Jiaheng Wang, Aimee A. Malzahn1, Jun Wu, Xuelian Zheng, Yong Zhang, Yiping Qi. CRISPR–Cas12b enables efficient plant genome engineering. Nature Plants. 2020, 6:202–208
5. Zhong, Zhaohui#; Liu, Shishi#; Liu, Xiaopei; Liu, Binglin; Tang, Xu; Ren, Qiurong; Zhou, Jianping; Zheng, Xuelian; Qi, Yiping*; Zhang, Yong*; Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing, Rice, 2020, 13: 8.
6. Tang, Xu#; Ren, Qiurong#; Yang, Lijia; Bao, Yu; Zhong, Zhaohui; He, Yao; Liu, Shishi; Qi, Caiyan; Liu, Binglin; Wang, Yan; Sretenovic, Simon; Zhang, Yingxiao; Zheng, Xuelian; Zhang, Tao*; Qi, Yiping*; Zhang, Yong*; Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing , Plant Biotechnology Journal, 2019, 17
7. 1431-1445. (7) Malzahn AA, Tang X, Lee K, Ren Q, Sretenovic S, Zhang Y, Chen H, Kang M, Bao Y, Zheng X, Deng K, Zhang T, Salcedo V, Wang K, Zhang Y, Qi Y. Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis. BMC Biology, 2019, 17(1):9.
8. Qiurong Ren, Zhaohui Zhong, Yan Wang, Qi You, Qian Li, Mingzhu Yuan, Yao He,Caiyan Qi, Xu Tang, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang. Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front. Plant Sci, 2019, 10:1173.
9. WANG Bo, ZHONG Zhaohui, ZHANG Huanhuan, WANG Xia, LIU Binglin, YANG Lijia, HAN Xiangyan, YU Deshui, ZHENG Xuelian, WANG Chunguo, SONG Wenqin, CHEN Chengbin, ZHANG Yong. Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice. Rice Science, 2019, 26(2): 98-108.
10. Zhou J, Xin X, He Y, Chen H, Li Q, Tang X, Zhong Z, Deng K, Zheng X, Akher SA, Cai G, Qi Y, Zhang Y. Multiplex QTL editing of grain-related genes improves yield in elite rice varieties. Plant Cell Reports, 2019, 38: 475–485.
11. Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang. Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9. 2019, Plant Genome Editing with CRISPR Systems: Methods and Protocols, Methods in Molecular Biology, vol. 1917
12. Zheng X, Yang L, Li Q, Ji L, Tang A, Zang L, Deng K, Zhou J, Zhang Y, MIGS as a Simple and Efficient Method for Gene Silencing in Rice, Front. Plant Sci, 2018, 9:662.
13. Tang, Xu; Liu, Guanqing; Zhou, Jianping; Ren, Qiurong; You, Qi; Tian, Li; Xin, Xuhui; Zhong, Zhaohui; Liu, Binglin; Zheng, Xuelian; Zhang, Dengwei; Malzahn, Aimee; Gong, Zhiyun; Qi, Yiping; Zhang, Tao; Zhang, Yong. A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice. Genome Biology. 2018, 19:84.
14. Zhong, Zhaohui; Zhang, Yingxiao; You, Qi; Tang, Xu; Ren, Qiurong; Liu, Shishi; Yang, Lijia; Wang, Yan; Liu, Xiaopei; Liu, Binglin; Zhang, Tao; Zheng, Xuelian; Le, Ysa; Zhang, Yong; Qi, Yiping, Plant genome editing using FnCpf1 and LbCpf1 nucleases at redefined and altered PAM sites, Molecular Plant, 2018, 7:999-1002.
15. 周建平,吉琳艺,何瑶,郑雪莲,罗樊,蔡光泽,张勇.利用CRISPR-Cas9系统高效创制水稻品系“17318”的OsGW2、OsGN1a基因定向突变体.西昌学院学报(自然科学版), 2018, 32:1-5.
16. Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng XL, Voytas DF, ZhongZH, Chen YY, Ren QR, Li Q, Kirkland ER, Zhang Y, Qi Y, A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants, Nature Plants, 2017, 3(7): 17018
17. Zhou JP#, Deng KJ#, Cheng Y, Zhong ZH, Tian L, Tang X, Tang AT, Zheng XL, Zhang T, Qi YP, Zhang Y*, CRISPR-Cas9 Based Genome Editing Reveals New Insights into MicroRNA Function and Regulation in Rice, Frontiers in Plant Science, 2017, 8:1598
18. Liu Y, Sun G, Zhong ZH, Ji LY, Zhang Y, Zhou JP, Zheng XL*, Deng KJ*. Overexpression of AtEDT1 promotes root elongation and affects medicinal secondary metabolite biosynthesis in roots of transgenic Salvia miltiorrhiza. Protoplasma, 2017, 254(4):1617-1625
19. Wei T, Deng K, Zhang Q, Gao Y,Liu Y, Yang M, Zhang L, Zheng X, Wang C, Liu Z, Chen C* and Zhang Y*. Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza. Front Plant Sci. 2017, 8: 52.
20. 刘玉; 孙耿; 仲昭晖; 郑雪莲(*); 邓科君(*), 烟草中过表达丹参SAMDC基因增强其抗旱性（英文）, 农业生物技术学报, 2017.3, (05)：729~738.
21. Tang Xu*, Zheng Xuelian*, Qi Yiping, Zhang Dengwei, Cheng Yan, Tang Aiting, Daniel F. Voytas, Zhang Yong, A single transcript CRISPR-Cas9 system for efficient genome editing in plants, Molecular Plant, 2016, 9(7): 1088~1091
22. Zheng Xuelian, Yang Shixin, Zhang Dengwei, Zhong Zhaohui, Tang Xu, Deng Kejun, Zhou Jianping, Qi Yiping, Zhang Yong, Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism, Plant Cell Reports, 2016, 35(7): 1545~1554
23. Zheng Xuelian, Zhou Jianping, Zang Lili, Tang Aiting, Liu Dongqing, Deng Kejun, Zhang Yong, Genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient. Genetics and Molecular Research, 2016, 15(2):
24. Deng K，Zang L，Lan X，Zhong Z，Xiong B，Zhang Y，Zheng X(*). Antioxidant components from Cape Gooseberry. Journal of Food Processing and Preservation. 2016 40(5): 893-898.
25. Deng K，Lan X，Sun G, Ji L, Zheng X(*). Determination of sulfonamide residues in chicken liver using high-performance liquid chromatography. Food Analytical Methods. 2016 9,(12): 3337–3344
26. Zheng Xuelian, Li Qian, Liu Dongqing, Zang Lili, Zhang Kaiyue, Deng Kejun, Yang Shixin, Xie Zhengyang, Tang Xu, Qi Yiping, Zhang Yong, Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum. Plant Cell Reports, 2015, 34(11): 1873~1884
27. Levi G. Lowder, Zhang Dengwei, Nicholas J. Baltes, Joseph W. Paul III, Tang Xu, Zheng Xuelian, Daniel F. Voytas, Tzung-Fu Hsieh, Zhang Yong, Qi Yiping, A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation, Plant Physiology, 2015, 169: 971~985
28. Liu Yu, Yang Shixin, Cheng Yan, Liu Dongqing, Zhang Yong, Deng Kejun, Zheng Xuelian*, Production of Herbicide-Resistant Medicinal Plant Salvia miltiorrhiza Transformed with the Bar Gene. Appl Biochem Biotechnol, 2015, 177: 1456~1465
29. Shan Qiwei, Wang Yanpeng, Chen Kunling, Liang Zhen, Li Jun, Zhang Yi, Zhang Kang, Liu Jinxing, Voytas Daniel F, Zheng Xuelian, Zhang Yong, Gao Caixia, Molecular Plant, 2013, 6(4): 1365~1368
30. Zhang Mi*, Zheng Xuelian*, Song Shuiqing, Zeng Qiwei, Hou Lei, Li Demou, Zhao Juan, Wei Yuan, Li Xianbi, Luo Ming, Xiao Yuehua, Luo Xiaoying, Zhang Jinfa, Xiang Chengbin, Pei Yan, Nature Biotechnology, 2011, 29(5): 453~45
联系方式
电子邮箱：zhengxl@uestc.edu.cn